gatherVCF¶
This function is used for gather VCF files into one file using gatk.
Note
This function is calling gatk GatherVcfs, please install GATK before using.
McKenna, Aaron, et al. “The Genome Analysis Toolkit: a MapReduce framework for analyzing next-generation DNA sequencing data.” Genome research 20.9 (2010): 1297-1303.
Parameters¶
gatherVCF(vcfInput=None, outputdir=None,
upstream=None, stepNum=None,
threads=1, verbose=False, **kwargs)
vcfInput: list, vcf Input files, you can give me as this: [[‘samp1-1.vcf’, ‘samp1-2.vcf’…],[]…].
outputdir: str, output result folder, None means the same folder as input files.
threads: int, how many thread to use.
stepNum: int or str, step flag for folder name.
upstream: upstream output results, used for pipeline. This parameter can be True, which means a new pipeline start.
verbose: bool, True means print all stdout, but will be slow; False means black stdout verbose, much faster.
Warning
We recommend using this function in SNV detection. For a detailed tutorial, please see SNV detection tutorial.
Example usage:
from cfDNApipe import *
import glob
pipeConfigure(
threads=60,
genome="hg19",
refdir=r"path_to_reference/hg19",
outdir=r"path_to_output/snv_output",
data="WGS",
type="paired",
build=True,
JavaMem="10G",
)
# just set build=True to finish all the works
Configure.snvRefCheck(folder="path_to_reference/hg19/hg19_snv", build=True)
# see all the SNV related files
Configure.getConfig("snv.folder")
Configure.getConfig("snv.ref")
# indexed bam files after remove duplicates
bams = glob.glob("path_to_samples/*.bam")
res1 = addRG(bamInput=bams, upstream=True)
res2 = BaseRecalibrator(
upstream=res1, knownSitesDir=Configure.getConfig("snv.folder")
)
res3 = BQSR(upstream=res2)
res4 = getPileup(
upstream=res3,
biallelicvcfInput=Configure.getConfig('snv.ref')["7"],
)
res5 = contamination(upstream=res4)
# In this step, files are split to chromatin
res6 = mutect2t(
caseupstream = res5,
vcfInput=Configure.getConfig('snv.ref')["6"],
ponbedInput=Configure.getConfig('snv.ref')["8"],
)
#
res7 = filterMutectCalls(upstream=res6)
# put all chromatin files together
res8 = gatherVCF(upstream=res7)
# split somatic mutations
res9 = bcftoolsVCF(upstream=res8, stepNum="somatic")
# split germline mutations
res10 = bcftoolsVCF(
upstream=res8, other_params={"-f": "'germline'"}, suffix="germline", stepNum="germline"
)